ABSTRACT
To retrospectively analyze the application trend of cohort study in the field of liver cancer in past 27 years and to look forward to the future development trend. Chinese and English papers reporting the cohort studies of liver cancer conducted in the mainland of China since 1991 were included. The literature management software was used to analyze the publication time, institution, type and objective of the studies and the follow-up performance. Statistical analysis was carried out by using SPSS 21.0. The number and quality of the papers reporting liver cancer-related cohort studies increased significantly in past 27 years and a core English journal group of this field has formed. The average annual growth rates of Chinese and English papers published were 20.4 and 35.2 respectively. About 52.3 of the Chinese papers and 73.5 of the English papers were published in past five years and the quality of these papers was high. The Chinese papers published on Chinese core journals accounted for 49.2, and the English papers published on SCI periodicals (IF>3) accounted for 47.3. For the study objective, those published on the domestic journals mainly focused on the pathogenesis of hepatoma (41.5), studies with large sample size was the common form. The SCI studies mainly focused on the prognosis of liver cancer (40.7). High-quality SCI papers are more interested in the studies of prognosis, and survival analysis was the common form. The application of cohort study in the field of liver cancer gradually increased in China. Large-scale study and prognosis analysis were conducted commonly. However, it is necessary to further improve the researchers' understanding of cohort study, improve the follow-up quality, and increase the application of scientific evaluation methods, such as survival analysis, for the better solving of clinical problems.
ABSTRACT
Objective To investigate the incidence of hyperuricemia in military pilots and the relationship between hyperuricemia and other related risk factors of cardiovascular diseases. Methods A total of 2563 pilots, who had undergone a regular physical examination in the Air Force General Hospital from 2005 to 2010, participated in the current study. The participants were divided into high serum uric acid (UA) group (hyperuricemia group, UA >420μmol/L, n=294) and normal UA group (n=2269). The incidence rates of hypertension, hyperglycemia, and hyperlipemia were compared between these two groups. The dependent variable was serum UA level of the pilots. Other indices included the total cholesterol (CHO), triglycerides (TG), high density lipoprotein (HDL), low density lipoprotein (LDL), fasting blood glucose (FBG), systolic blood pressure (SBP), diastolic blood pressure (DBP), smoking, body weight, and flight time as independent variable. Logistic multivariate regression analysis was conducted to determine the relationship between UA level and these indexes. Results The incidence rates of hypertension, hypercholesterolemia, hypertriglyceridemia, low HDL, and high LDL were significantly higher in hyperuricemia group than in normal UA group (P<0.01). The Multiple logistc-regression analysis suggested that high UA level was closely related to TG (OR=1.637, P<0.01), DBP (OR=1.025, P<0.01) and body weight (OR=1.046, P<0.01). Conclusion The serum UA of pilots is closely related to the risk factors of other cardiovascular diseases.
ABSTRACT
Total glucosides of Picrorhiza scrophulariiflora (TGP) is the active principal in Picrorhiza scrophulariiflora which has antioxidant effect. Since oxidative stress plays a key role in diabetic nephropathy, we investigated the effects of TGP on oxidative stress in bovine glomerular mesangial cells (MC) induced by prolonged high glucose. Bovine glomerular mesangial cells were cultured and passages 2-3 were used for the experiment. Mesangial cells were cultured in high glucose medium, and treated with TGP for 3 weeks. Then collagen IV excreted by mesangial cells were detected, and the percentages of cell cycle were observed by flow cytometry technique. The levels of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and [Ca2+]i were measured by flow cytometry after loaded with fluorescent probe DCFH-DA, Rh123 and fluo-3-acetoxymethylest. TGP significantly decreased the excretion of collagen IV and cell hypertrophy induced by high glucose, reduced the levels of ROS and [Ca2+]i, and increased MMP. Therefore we conclude that TGP could protect mesangial cells against oxidative stress induced by high glucose.